HPLC.

• Microprocessor based gradient HPLC System with Quaternary pumps capable of working with multiple detectors namely RID, PDA and Fluorescent detector, PC and software and required pumping system.
1. Management System (Quaternary Pump) HPLC Pump:• High pressure mixing with on-line micro vacuum degasser
• Two Dual Piston in series
• Solvent Conditioning: 4 channel Vacuum degassing system.
• Plunger Serial Waste: Should be active and programmable
• Composition range:0-100%
• Composition precision: < 0.15% SD, at 1.0 ml/min
• Composition accuracy: < 0.35% Absolute
• Gradient Profile: multiple gradient curves
• Maximum Operation Pressure :8000 psi
• flow rate: (0.001– 9.999 ml/minute)
• Programmable upper and lower limits
• Flow Precision : Not more than ± 0.075 %
• Flow Accuracy: ±1%
• Delay Volume:120µl
• Custom Plumbing setup for a gradient system with tubing.                                           
 • Auto Sampler:
i. Should have overlapped injections.
ii. Should hold two well plate, 96 plus 10 additional 2 ml vials.
iii. Should hold 100 or more x 2-mL vials
iv. Should hold eppendorf tubes preferably
v. Carry over should be < 0.01ml.
vi. 0.1–100 µl standard injection range
vii. Precision typically < 0.25%, from 5-100 µl & 1% from 1-5 µl.                        
            2. Sample Injection System
• No. of sample vials distribution in different carousels: 100 vials or more and should be independent carousels.
• Sample temperature control: 4 to 40 degree C (heater/cooler)
• Time Programming capability and Oven On/ off
• Leak sensor for safety and leak detection

• Column Heater:
i. Within the range of 20 degree C – 60 degree C
ii. Column identification accessory should be included
• Preferably have capacity to hold 2 columns of 30 cm length simultaneously; Should also quote column switching valve for column auto regeneration                            3. Detectors:
i. Photodiode Array Detector (PDA detector)
• Wavelength range: 190 - 800nm.
• Light source: Pre-aligned, Deuterium lamp with one year warranty.
• Spectral Resolution: 1.2nm per photodiode with a total of 512 photodiodes, digital and optical (3D modes).
• Data Rate: Upto 80Hz
•  Digital Resolution: 1.2nm – 600nm (2D mode).
• Wavelength accuracy: +/_ 1nm.
• Linearity range: >5% at 2 AU Prolylparaben 257nm                                      
 •Baseline noise: 10.0 x 10-6 AU, at 254nm.
• Drift: < 1.0 x 10-3 AU/hour/°C, dry cell at 254nm.
• Sensitivity setting range: 0.0001 - 2.0000 AUFS (under software control).
• Filter setting range: 0, 0.1, 0.2, 0.5, 1, 2, 3
• Path length: upto 10mm
• Cell Volume: upto 8ul
• Pressure: upto 1000psi
• Wetted materials: 316 stainless steel, fused silica, Tefzel
• Auto threshold for peak purity along with peak purity software.
• The detector should have lamp optimization software.
• Mode of Operation: Scanning and detection at variable/fixed wave length.             ii. Scanning Fluorescence Detector
• Light Source: 150 W Xenon Lamp
• Wave Length Range:200-900 nm for both excitation and emission
• Wave Length Repeatability: 0.3 nm
• Cell volume: Approx. 8 micro lit
• Band with:  Approx. 20 nm
• Sensitivity S/N Raman:  Approx. >800nm

Automation: Software and manual controls. The detector should have lamp optimization software, Variable Scanning and analysis facility                              
     iii. Refractive Index Detector
• Measurement Range: 5.0 x 10-3 R/U max (SENS=1, SF=1) - 5.0 x 10 R/U min. (SENS-1024, SF=100)
• Flow Cell: Fused Quartz
• Cell Volume: 10µl
• Cell Pressure: 100 psi max. With built-in pressure relief valve
• Linear Dynamic Range: 5 x 10-3to 5 x 10 R/U
• Auto Zero Facility and Adjustment with refractive Index test samples.
• Automation: Software and manual controls.                                               
            The detector should have lamp optimization software, Variable Scanning and analysis facility                                                                                                                            iv. (UV) Detector
• Wavelength: Complete UV-VIS range
• Source: Deuterium and / or Tungsten
• Noise: Approx. ± 0.35x10-5 AU, dry cell 254 nm
• Drift:  Approx. <2x10-4 AU/hr.
• Linearity: Approx. 5 nm
• Accuracy  Approx. ± 1 nm
• Reproducibility: Approx. ± 0.1 nm
• Automation: Software and manual controls. The detector should have lamp optimization software, Variable Scanning and analysis facility       
      4. Column oven model: Temperature Range Ambient +4°C to 60°C
5. Columns
• C-18 - 250 x 4.6 mm
• C-8   250 x 4.6 mm
• C-18 - 250 x 20 mm
• C-8 - 250 x 20 mm
• Pre-column derivatisation kit for Amino Acids
• Bio suite C-18 PA-A 3 :m - 4.6 x 250 mm
• Protein pak - 7.8 mm x 300 mm

• Loop connecter/loop: Separable if any accidentally leak occurred
• Working Bench: Fabricated working bench vibration free for instrument.
• Water purification System (from tap water to ultra-pure water for HPLC)
11. Documentation
• User/Technical/Maintenance manuals to be supplied in English.  
• Certificate of calibration and inspection.  
• List of Equipments available for providing
calibrationand routine PreventiveMaintenance Support as per manufacturer documentation in service/technical manual.
• List of important spare parts and accessories with their part number and costing.  
• Compliance Report to be submitted in a tabulated and point wise manner clearlymentioning the page/para number of original catalogue/data sheet.
• Log book with instruction for daily, weekly, monthly and quarterly maintenance checklist.
12. Laboratory establishment
13. Training for operations and trouble shooting